Using SPOT-VGT NDVI data from 1999 to 2010, with the method of correlative analysis based on pixels, the response of vegetation to climate change in karst region of Guizhou Province was analyzed. The results showed that: from 1999 to 2010, the climate of the study area changed with a trend of being dry and cold and NDVI recovered. From the aspect of region, there was no significant correlation between NDVI and the climate; while from the aspect of pixels, NDVI was negatively correlated with the annual mean temperature and the annual precipitation, and the influence of annual mean temperature was less than that of the annual precipitation. As NDVI was negatively correlated with climate, the climate changing with a trend of dry and cold had an advantage of the whole vegetation NDVI recovery in the region. The correlation between vegetation NDVI and climate had a strong feature of regionalism in space, which further proved that the response of vegetation NDVI to climate change was strongly influenced by terrain.