Taking the land use data of Zhenjiang City in 1997-2009 as a case study, the land use structure dynamic evolution characteristics and regional differentiation rules of Nanjing-Zhenjiang-Yangzhou hilly area were analyzed and discussed by using Shannon entropy and spatial lorrenze curve. Some conclusions were drawn as follows:(1) From 1997 to 2009, the spatial distribution of different land use types in Zhenjiang City was unbalanced, specially, the distribution of arable land and land for residential areas and mining was more balanced, and the distribution of grassland and woodland was relatively concentrated;(2) Land use structure evolution had periodical characteristics, it can be divided into 3 phases: low speed expansion stage(1997-2000), speed-up expansion stage(2001-2004), steady development stages(2005-2009), the areas of cultivated land and residential and industrial land had significant correlation to the entropy, which could be regarded as the key factors influencing the entropy of land use structure in Zhenjiang City;(3) The land use structure evolution showed regional distribution rules, the urban information entropy was larger than the other counties of Zhenjiang City, and presents the declining trend, it indicated that urban land use structure was in the order of improvement gradually, however, other county revealed a rising trend, it indicated that the order of land use structure was declining.