Taking 28 counties(city or area) as evaluation units, the spatial differentiation of cultivated land intensive use in Nansihu Basin of Shandong Province was analyzed by adopting entropy and comprehensive index method combine with GIS in 2011. The results showed that the intensive use level of the cultivated land in Nansihu Basin revealed obvious regional differentiation characteristics which influenced by the natural, social and economic conditions of the regions. The intensive use level of the low hilly land in the east lake is significantly lower than that of the Yellow River flood plains in the west lake. Moreover, the measures to improve the intensive use level of cultivated land are different for different counties(city or area).