The microclimatic effects and habitat types of bryophytes were observed in karst world natural heritage nominated property of Shibing County in Guizhou Province. The results showed that bryophyte communities could affect the surrounding microclimate by lowering the air temperature and increasing the relative humidity. The microclimatic influencing sphere of of bryophytes was almost within a radius of 10 cm. The most significant microclimatic effects were found in open space. The results of paired-sample test showed that the difference of relative humidities between layer A(the surface of bryophyte community) and layer D(10 cm horizontally away from the bryophyte community) achieved very significant level(p<0.01), and that the temperature difference between layer A and D was statistically significant(p<0.05). Both the differences of relative humidities and temperatures between layer A and layer B(10 cm up the bryophytes) were statistically significant(p<0.05). The intensity level of the microclimatic effects depends on the water storing ability of the bryophytes.