With 3S technology, characteristics of land use/cover change and variations of ecosystem services value from the year from 2000 to 2010 in 14 national nature reserves in Jilin Province were analyzed with remote sensing image. Results showed that during the period of 2000-2010, the area of construction land, grassland and farmland increased, while the area of forest land, wetland, water body and unused land decreased; the dynamic rate of integrative land use change from 2000 to 2010 was 0.15%, and the spatial dynamic rate of the region was 0.43%; forest land changed with the lowest change rate compared with the regional average change rate. With the rate of 1.73%, ecosystem services value in nature reserves decreased by 9.95×10⁸ Yuan per year. Ecosystem services value of forest, wetland, water body and unused land decreased with the rate of 0.31%, 6.78%, 5.15% and 8.96%, respectively. Ecosystem services value of grassland and farmland increased with rate of 15.67% and 5.13%, respectively. Ecosystem services value of many land use types and multiple ecosystem services all decreased in different rates in the national nature reserves.