[Objective] To analyze the influence of land use and lithology on soil properties and saturated hydraulic conductivities (K_s).[Methods] The surface soil from limestone and dolomite slopes in karst region was took as the study objective, and Guelph parameter and soil laboratory analysis were used.[Results] (1) Under over grazing condition, soil bulk density and clay content increased and porosity and organic matter content decreased significantly on both of limestone and dolomite slopes. (2) Under undistured land use condition, K_s of limestone and dolomite soil was very high, the average value was 328.6 mm/h and 257.2 mm/h respectively. Compared to dolomite slope, the spatial distribution of K_s on limestone slope was more heterogeneities that coefficients of variations (C_v%), which changed between 90.71% and 95.62%. (3) Under the influence of over grazing, the K_s of limestone and dolomite soil decreased by 52.2% and 86.7% respectively, significantly lower than average values.