[Objective] To improve the studies on ecological conditions, utilization, and protection of forest resources, the soil spatial variation and the spatial distribution of sample sites in forest of Hu'nan-Jiangxi boundary mountain areas were analyzed.[Methods] The soil nutrient content was measured, and the comprehensive advantage of soil nutrients was evaluated by the changed Nemero formula, the soil spatial variability was evaluated by the coefficient of variation(CV).[Results] (1) Soils of the experimental region were characterized as acidic and had high values of soil organic matter(SOM) and soil total nitrogen(STN) generally, but were all lack of soil available phosphorus(SAP) and soil available potassium(SAK)nutrients, (2) With the rise of elevation level of hill, low mountain, middle mountain, the values of soil pH, SOM, STN, SAP and comprehensive fertility coefficient increased, but the spatial variation of soil pH, SOM and STN decreased. (3) According to the different forest types, the comprehensive advantage of soil nutrients were made as follow:shrubs> the mixed forest> Phyllostarchys pubescens forest> broad-leaved forest> coniferous forest, the order of the spatial variation were:shrubs> coniferous forest> P. pubescens forest> broad-leaved forest> the mixed forest. (4) All the soil nutrient indicators of natural forest, except the SAP, were higher than that of plantation.[Conclusion] The forest in middle mountain, mixed forest and natural forest have more potential to maintain good soil nutrient status in the study area.