[Objective] To research the effects of two seedlings[tissue culture seedling(P₁) and seed seedling(P₂)] of Eucalyptus grandis plantation on soil anti-erodibility.[Methods] Soil samples of 0-10c m, 10-20 cm and 20-30 cm were collected with ring-knife and plastic box to determine the index as soil bulk density, non-capillary porosity, capillary porosity, total porosity, soil aeration porosity and water stable aggregates content, etc.[Results] The soil bulk density in the two seedling plantation lands increased at the downward soil profiles, while total porosity and soil aeration porosity decreased with the increase of soil depth. The soil bulk density in P₁ was significantly lower than that in P₂. The total porosity and soil aeration porosity in P₁ were significantly higher than those in P₂. Water stable index, soil erosion resistance, aggregate condition, aggregate degree and structure coefficient in the two kinds of plantations decreased with the increase of soil depth, while the soil dispersion rate and dispersion coefficient increased. The water stability and resistance to corrosion index were both significantly different at the 0.05 level. Soil erosion resistance and structure coefficient in P₁ were higher than those in P₂, while the dispersion coefficient was lower than that in P₂. Contents of water-stable aggregate in all size and MWD(mean weight diameter) in both plantations decreased with the increase of soil depth, while soil structure damage rate increased. Contents of water-stable aggregate in different size(>5 mm, 5~2 mm, 0.5~0.25 mm, >0.5 mm, >0.25 mm) and MWDS in P₁ were higher than those in P₂, while the structure damage rate, and water-stable aggregate in sizes of 2~1 mm and 1~0.5 mm in P₁ were lower than those in P₂.[Conclusion] Soil anti-erodibility in P₁ was higher than that in P₂, in the present study condition.