[Objective] To analysis the dynamic variation of slope runoff and sediment yield of three different land use types in the past three years, and provide a theoretical basis for sustainable management of Eucalyptus Grandis×E. Urophylla.[Methods] A located observation on runoff plots of three different land use types of Eucalyptus plantation, shrubbery and abandoned land of Pu'er City in Yunnan Province was conducted in the past three years, to analyze the runoff and sediment yield of the different land use types.[Results] The rainfall of the study area was observed concentrated in June to October from 2010 to 2012.The rainfall of study area gradually reduced in the past three years. The runoff observed in the three runoff plots ranked as:2012<2010<2011. The runoff in 2012 reduced by 52.28% and 40.92%, as compared to the value in 2011 and 2010, respectively. The runoff observed from June to October of the three land use types in 2010-2012 ranked as:Eucalyptus plantation< shrubbery< uncultivated land. The runoff in that period of Eucalyptus plantation reduced by 25.01% and 30.86%, respectively, compared with shrubbery and abandoned land. The yearly sediment rank observed in the three plots was 2012<2010<2011.The observed value in 2012 reduced by 41.83% and 18.49%, respectively, as compared to the sediment in 2011 and 2010. Sediment and the total annual runoff volume varied consistently in the past three years. Eucalyptus plantation reduced sediment yield by 47.56% and 50.69%, respectively, against the shrubbery and abandoned land.[Conclusion] Eucalyptus plantation has the best effect of soil and water conservation.