[Objective] This paper aims to demonstrate the spatial autocorrelation and the pattern of soil temperature, and to find the main environmental factors.[Methods] Classical statistics and 3S technology were used.[Results] The spatial distribution of soil temperature in different soil depth showed a general highly positive spatial autocorrelation and had obvious spatial clusters, nearly no spatial isolated distribution was found. The impacting factors of spatial distribution of soil temperature in different soil depth ranked as:soil thickness, slope, vegetation coverage and altitude.[Conclusion] The influence of soil thickness on soil temperature, essentially, is the comprehensive reflection of karst environment. Terrain factors, like slope, elevation and so on, are the main factors influencing soil inversed temperatual structure. In addition, the spatial heterogeneity of soil temperature also been affected by "covering" or "heat preservation effect" of plants.