[Objective] To explore the quality of the Lonicerae flos flower in different levels of soil trace elements in order to confirm the optimum planting years of Lonicerae flos in Suiyang County, Guizhou Province. [Methods] Field investigation and laboratory analysis of a combination of methods were used. [Results] In the soil, pH value was from 4.67 to 6.01, and the average contents of Cu, Mo, Mn, and Zn were from 17.17 to 37.50 mg/kg, 1.52 to 2.24 mg/kg, 444.6 to 544.26 mg/kg, 102.06 to 114.39 mg/kg, respectively. And in the buds of Lonicerae flos, the average content was from 6.91 to 14.52 mg/kg, 0.16 to 0.24 mg/kg, 30.06 to 79.36 mg/kg, 12.73 to 23.54 mg/kg for Cu, Mo, Mn, and Zn, respectively. At the same time, the chlorogenic acid content was from 2.988% to 7.840% for Lonicerae flos planted in different years. [Conclusion] The contents of Zn and Cu in soil increase along with the increasing of planting years, but the contents of Mo and Mn decrease. The content of chlorogenic acid is at a higher level and the plants are growing well in 11 to 15 planting years. Trace elements contents in flowers show an upward tendency as a whole in the course of their planting years. The growth of stem and leaf of Lonicerae flos is greatly favored by Mn in soil with an additional favor from Cu and Zn, which helps the botanic growth and promotes the synthesis of chlorogenic acid.