[Objective] The effects of two ecotypes(farmland and mining) of accumulator plants intercropping with cherry seedlings on soil enzyme activity under cadmium stress were examined in order to provide the reference for cherry production in cadmium pollution area. [Methods] Tianxin cherry and Naweng cherry were intercropped with two ecotypes of cadmium accumulator Conyza canadensis, Solanum nigrum and Digitaria sanguinalis, respectively, and the soil catalase, urease and invertase activities were determined. [Results] When Tianxin cherry intercropped with mining ecotype of D. sanguinalis, the soil catalase and urease activities reached to the highest values, which were improved by 41.07% and 57.53% than monoculture of Tianxin cherry, respectively. When Naweng cherry intercropped with farmland ecotype of C. canadensis, the soil catalase activity reached to the highest value, and the soil urease activity reached to the highest value when intercropped with mining ecotype of D. sanguinalis. When cherry seedlings intercropped with two ecotypes of accumulator plants, only Tianxin cherry intercropping with farmland ecotype of C. canadensis, Naweng cherry intercropping with mining ecotype of D. sanguinalis and Naweng cherry intercropping with farmland ecotype of D. sanguinalis improved the soil invertase activity. [Conclusion] Under cadmium stress, the soil enzyme activity of cherry could be improved by intercropping, and the best material was mining ecotype of D. sanguinalis, and followed by farmland ecotype of C. canadensis.