[Objective] The effect of tourist trampling on the enzyme activity of Dongtai meadow in Wutai Mountain was investigated to provide a scientific basis for the development, protection, management of Wutai tourism resources. [Methods] Based on the combination of field sampling investigation and indoor analysis method. [Results] (1) Under the different levels of different altitudes, tourist trampling had the significant inhibitory effect on the soil urease activity and invertase activity, and they showed the sequence of no stampede areas > trampling slight areas > trampling serious areas; soil urease activity and invertase activity in the surface layer(0-20 cm) > soil urease activity and invertase activity in the subsurface layer(20-40 cm); (2) At the different altitudes, the soil urease activity and invertase activity in two layers of the trampling serious areas were different prominently from the no stamped areas, but invertase activity in trampling slight areas showed no significant difference compared with no stamped areas; (3) At different altitudes, catalase activity, phosphatase activity of stampede serious soil surface layer(0-20 cm) and the subsurface layer(20-40 cm) were significantly lower than those without trampling zone(p<0.05); soil catalase activity, phosphatase activity of trampling slight soil surface layer(0-20 cm) had no significant difference compared with those in the no stamped areas. [Conclusion] The soil enzyme activity of Dongtai meadow in Wutai Mountain had been destroyed, and it is necessary to take measures to protect Dongtai meadow of Wutai Mountain from being destroyed.