[Objective] Studying the variation of ecological services value in Anhui Province in order to provide a support for regional sustainable development. [Methods] Three phases of land use data of Anhui Province were used to calculate the ecological service value per unit area of different land use types and their areas. Ecological services value, contribution rate of ecological services value of different land use types resulted from their changes, and ecological service value of each function in different years were respectively analyzed from 2000 to 2010. [Results] The main land use types were forest land and cultivated land, and the following types were construction land and unused land. In 2000, 2005, 2010, the total values of ecosystem services were 53.231, 53.759, 53.135 billion yuan, respectively. The contribution of six types of land use ranked as: forest land > cultivated land > water area > grassland > unused land > construction land. Forest land and construction land contributed mostly to the changes of ecosystem services value. Functions of ecological service value were ordered as follows: maintaining biodiversity > keeping soil > climate regulation > gas regulation > raw materials production > providing aesthetic landscape > food production > waste treatment > hydrology regulation. [Conclusion] The ecosystem services value in the study area was stable largely, of which the forest lands had the largest contribution to the ecological services value, and the construction land was the main holdback force of the growth of the ecological services value in the study area.