[Objective] To study the impacts of micro-topography on plant functional groups, biomass and soil water content in order to provide theoretical reference for environmental construction and reasonable development in the Hunshadake sandy land area.[Methods] The investigations were conducted in sandy land, in Inner Mongolia grassland ecosystem research station(IMGERS). Soil water content, biomass and plant functional groups were investigated under different micro-topographic conditions.[Results] The bottom of hill was dominated by perennial grass, while the top of hill was dominated by perennial grasses and annual or biennial herb. Shrub, subshrub and perennial grasses were distributed on the north-facing slopes, while shrub, subshrub, perennial grasses and annual or biennial herb were distributed on the south-facing slopes. The soil water content(0-50 cm) was showed as follows:bottom of the slope >north-facing slope >south-facing slope >top of the slope, and same trend was showed in biomass. Coefficient of variation of soil water content(0-50 cm) was the greatest at the top of the slope, while it was the lowest at the bottom of the slope. Aedbetween biomass and soil water content, and significant linear correlation existed between biomass an soil water content in top soil(0-5 cm), soil at the depth of 5-10 cm and 20-30 cm(p<0.01).[Conclusion] Micro-topography influences soil water content and florisitics, and there is a significant positive correlation between soil water content and biomass.