[Objective] Monitoring sites are the bases of soil and water conservation monitoring. Scientific and reasonable sites layout would directly impact on the accuracy of monitoring data as well as authority. Hence, the spatial localization optimization of monitoring sites was researched in order to provide some supports for effective operation of conservation system and reasonable decision of associated policy.[Methods] Through data collection of soil erosion, combined with GIS spatial analysis, soil erosion intensity classification in Hubei Province was accessed. The theory of scale space was used to optimize the location of soil and water conservation monitoring sites, after that the results were verified using the Thiessen polygon method.[Results] 86 monitoring sites were arranged in the whole province, including 12 monitoring sites at grade 1st scale, 26 monitoring sites at level 2nd scale, 48 monitoring sites at level 3rd. Thiessen polygon verification results showed that the optimized sites were reasonable.[Conclusion] The surrounding hills of Danjiangkou Reservoir, the farmland zone of Dahong Mountain and Nanyang basin, the water conservation districts of Hubei and Chongqing mountainous area were serious in soil erosion. More monitoring sties need to be settled in this area. The surrounding farmland area of Jianghan plain had less soil erosion, and where the amount of soil erosion monitoring sites can appropriately be reduced.