[Objective] The comprehensive effects of land use change on eco-environment during 1995-2014 were assessed to provide a support for land use management and urban eco-environment governance.[Methods] Based on the TM image data of central district of Hangzhou City in 1995, 2000, 2005, 2010 and 2014, methods of ecosystem service value, carbon emission and landscape pattern assessment were used.[Results] The ecosystem service value in Hangzhou City was 3.24 billion Yuan in 1995 and 2.40 billion Yuan in 2014, which decreased by 26%. The ratio of ecosystem service value to GDP decreased from 8% in 1995 to 0.5% in 2014. The carbon emission increased from 4.26×10⁶ t in 1995 to 1.51×10⁷ t in 2014, and the carbon sink decreased from 7.22×10⁵ t in 1995 to 6.49×10⁵ t in 2014. The social cost of carbon emission was about 14.5 billion Yuan in 2014.Areas with low ecosystem service value spread its coverage from city center to periphery. Landscape pattern analysis showed that, in the past 20 years, land use had tendencies of decreasing fragmentation, increasing connectivity and decreasing diversify.[Conclusion] In the study area, the sharp decrease of ecosystem service value can be attributed to the decrease of forest land and water body, and the increase of construction land. The increase of carbon emission linked with land use change was a consequence of increased carbon emitted from construction land and decreased sink of forest land. Land use transformation caused the change of landscape pattern remarkably, and the spatial distribution of ecosystem service value was uneven.