[Objective] The objective of this study is to explorer soil respiration features and its responds to soil temperature and soil moisture under different vegetation in order to provide references for the accurate estimation of payment changes and balance of soil organic carbon. [Methods] Soil respiration rate(R_s) of four typical succession communities including grassland, thin shrub-grassland, shrub land and forest land were observed by using LI-8100 soil carbon flux measurement system to investigate the influences of soil temperature(T) and soil moisture(W) on soil respiration in typical karst regions of Guizhou Province. [Results] (1) The seasonal variations of soil respiration rate of four vegetation types showed a similar unimodal distribution, with the peak occurred in summer and depression occurred in winter. The range of soil respiration rate in four vegetation types was 0.73~1.21,1.20~1.48,1.54~2.41 and 1.86~2.95 μmol/(m²·s), respectively. The average soil respiration rate was 1.65, 2.76, 2.45 and 3.43 μmol/(m²·s) in the observation period. (2) Univariate model indicated that soil temperature and moisture could explain soil respiration variation by 72.37% and 43.9%, respectively. Double factor model suggested that soil temperature and moisture together could explain 81.5%~91.2% of the seasonal variations in R_s. (3) The temperature dependence of soil respiration (Q₁₀) has been widely used in estimating soil respiration rate. Soil temperature and moisture were both negatively correlated to Q₁₀ of four vegetation types. [Conclusion] Soil temperature and moisture together influenced soil respiration rate and Q₁₀ values, and when the soil moisture was too high or too low, the effect of temperature was weakened, and the effect of the soil moisture reinforce.