[Objective] The aim of this study is to explore the variation characteristics of the micro-topography during the splash erosion under the rainfall intensity of 60 mm/h in order to provide the scientific basis for layout of the soil and water conservation farming pattern in loess slope land. [Methods] Four common tillage treatments (artificial backhoe, artificial digging, contour tillage and rake wear leveling) were prepared in the loess region. Artificial rainfall, laser scanning and GIS technology were adopted to generate high precision M-ΔDEM which can reflect the real variation of tillage slopes. [Results] Different tillage slopes showed low spatial variability, and its spatial pattern was mainly affected by tillage measures rather than rainfall. The rainfall in splash erosion had a general flattening effect on the micro-topography, and different tillage slopes showed an erosion balance within the scale of 1~1.3 m. Micro-topography has strong retarding effect on the splash erosion. The artificial digging and contouring tillage had a better soil and water conservation effect. [Conclusion] Micro-topographic stream with its fractal dimension can well reflect the complex characteristic of micro-topographic, and tillage treatments control the spatial and temporal variation of micro-topographic during splash erosion process.