[Objective] The objective of this study is to investigate the characteristics of land use landscape and its eco-environmental effects in northeast agriculture and forestry interlaced zone in order to provide scientific basis for resource utilization and ecological environment protection. [Methods] Based on the method of landscape ecology and 3S technology, remote sensing images in 1991, 2001, 2007 and 2013 were used, and landscape index calculation and model analysis were employed to study the evolution of land use landscape pattern and ecological service value. The driving forces of land use landscape evolution were also investigated. [Results] During the period from 1991 to 2013, landscape indexes varied as a "V" or "Λ" shape. The landscape indexes and diversity indexes decreased, while patch average area, sprawl and contagion indexes increased. The landscape fragmentation decreased, and the stability and diversity of landscape were damaged. The total value of ecosystem services decreased initially, and then increased continuously and reached to 5 242 995.5 thousand yuan in 2013. Forest was the greatest landscape type, which contributed above 40% for the total value of ecosystem services. [Conclusion] Land-use landscape and the value of ecosystem services changed greatly in the study area during the past 22 year, and population increase, economic development and government policy were the main driving factors.