[Objective] This article explored the possibility of the application of spatial autocorrelation method in the soil and water conservation zonation in order to provide guidance for the future work. [Methods] Taking the black soil region of the Northeast China as the study area, this paper used the method of spatial autocorrelation to analyze the distribution characteristics of the climate, landform, soil, plant and other factors in the study area and then derived dry and wet climate regionalization, geomorphic regionalization and soil regionalization separately. Finally, the soil and water conservation zonation of the black soil in the northeast of China was got by using the geometrical packing method. [Results] The study area was divided in to 10 synthetic units by overlay analysis. Adjusted according to the distribution characteristics of the plant, rainfall and geographical factors, the study area was then summarized into 8 classes, 2 zones finally. [Conclusion] The results showed that the zonation result is comparatively accurate, and this method is not only practical but also improve work efficiency.