[Objective] The effect of mixing two plant fiber into vegetation-compatible concrete on its shearing strength under the condition of freezing and thawing was studied, aimed to provide a scientific basis for the application of vegetation-compatible concrete based ecological protection technology in the alpine region. [Methods] According to slow freezing method in relevant test standards(GB/T50082-2009), 2 factors and 3 levels of comprehensive cross test was conducted. [Results] For the fiber unincorporated vegetation-compatible concrete, the frozen-thawed cohesion value of vegetation-compatible concrete was reduced remarkably as compared with that without freeze-thaw cycle, whereas no obvious change of internal friction angle value was observed. After 5 frozen-thawed cycles, the fiber addition made the internal friction angle value of vegetation-compatible concrete increased by an average of about 5°. The internal friction angle variation was not significant with the increase of fiber addition. However, cohesion firstly increased and then decreased with increasing fiber content, which implied that the contribution of fiber reinforcement to cohesion has a critical content. Palm fiber was proved more effective on cohesion than hemp fiber did as their content varied. [Conclusion] Fiber can improve the ability of vegetation-compatible concrete to against freezing and thawing. The reasonable proportion of palm and hemp fiber was 0.6% and 0.6%.Fiber added vegetation-compatible concrete with the above testified materials and proportion is applicable and has the market prospection.