[Objective] Land ecological sensitive zoning was comprehensively analyzed under different land use type to provide reference for regional land use planning. [Methods] Yixing City, a typical city in Southern Jiangsu Province was taken as an example, its land use ecological sensitive zones were divided according to the zones of soil and water loss sensitivity and biodiversity preservation priority using USLE model and landscape security pattern. [Results] The ecological sensitivity in Yixing City was thought relatively low. The zones with extremely high sensitivity accounted only for 7.87% of the total area, the zones with high sensitivity, moderate sensitivity and low sensitivity covered 23.96%, 35.71% and 32.46%, respectively. The zones with extremely high sensitivity were clustered together in space. Sensitivity declined gradually from the southern hill district to the northern plain district. [Conclusion] The application of USLE model combined with landscape security pattern can comprehensively recognize the factors that influence ecological sensitivity, and quantitatively grade the land use ecological sensitive zones.