[Objective] The objective of this study is to analyze the effects of rainfall intensity and land use on loss of organic matter in southwest coteau in Danjiangkou reservoir catchment and to provide help for preventing and controlling non-point source pollution and soil erosion of the region. [Methods] Artificial rainfall experiments, two-factor variance analysis method was adopted to investigate nutrient loss in different land use types in Danjiangkou reservoir catchment and the topsoil samples were collected from five common land use types to analyze the organic matter under six levels of rainfall intensity. [Results] (1) The rainfall intensity had significant effects on the loss of organic matter in runoff while land use types had little impact on it, the higher the rainfall intensity is, the more the organic matter loss. (2) Both rainfall intensity and land types had obvious influence on the organic matter content in sediment, increasing with the increase of rainfall intensity. The organic matter content in the sediment from farmland (terraces and hilly land) was higher than that from forest and grass land, which indicated the better effects of soil and water conservation of forest and grass land. (3) The regression analysis between the organic matter content in runoff and sediment with rainfall intensity showed that polynomial fitting gives best performance(except sediment fitting of shrubby grassland). [Conclusion] The most nutrients were rich in terraces soil, which could keep the nutrients and soil as a kind of soil conservation measures. Less nutrients were lost in woodland and grassland because of their better soil structure, which could reduce nutrient loss and soil erosion.