[Objective] The nutrient loss characteristics in different land use types were studied in southwest mountainous areas of He'nan Province to provide scientific basis for regional land planning, and for the prevention and control of agricultural non-point source pollution, water loss and soil erosion. [Methods] Five kinds of common farmland were selected in Taozhuanghe basin and Tiewahe basin, and wherein the nutrients concentrations in runoff were monitored and the nutrients loss was estimated by SCS model in normal flow year from different fields. [Results] The nutrients contents in all kinds of farmland were coincided with their loss observed in normal flow year, but were different in different catchment. In Taozhuanghe basin, COD was the most, nitrogen fertilizer was moderate and phosphate fertilizer was the least; while in Tiewahe basin, nitrogen fertilizer was the most, COD was moderate and phosphate fertilizer was the least. This indicated organic and nitrogen fertilizer were more preferably used in Taozhuanghe basin; nitrogen and ^*organic fertilizer were more frequently used in Tiewahe basin. Nutrients loss varied in different fields, in Taozhuanghe basin the rank was: herbs > sesame > corn > peanut > orchard; In Tiewahe basin, it was: vegetable > corn > slope > woodland > orchard. Nutrients loss was not only related to fertilizer type and application quantity, but also to the condition of underlying surface, such as slope, crops, etc. [Conclusion] It is necessary that the crop structure should be adjusted and the traditional fertilization custom should be changed according to local conditions to reduce nutrient loss and non-point source pollution.