[Objective] Studying the spatio-temporal variations of water storage in He'nan Province to provide a theoretical basis for the rational planning of agricultural development. [Methods] The spatio-temporal variations of terrestrial water storage changes(TWSC) of He'nan Province were derived from GRACE during 2002-2014, and the influence factors were analyzed. [Results] (1) The annual variation of TWSC in He'nan Province presented a cosine-shaped curve, in a loss state between January and July, and getting surplus between August and December. The lag time between the peaks of TWSC and precipitation was two months. (2) He'nan TWSC presented a decreasing trend during the observation period at a rate of-6.34 mm/a. Geographically, the reduction rate of water reserves was higher at the south part than that at the north part. [Conclusion] Through quantitatively calculation of the annual variation ratio of total water consumption of human activities and TWSC, it can be concluded that about 1/3 variation of water storage in He'nan Province is contributed by human activities, and the other part, about 2/3 of the variation came from natural factors.