[Objective] This paper were to demonstrate the defects potentially existed in present evaluation index system and diagnosis methods of the rural ecological civilization construction level from farmers' perspective.[Methods] The evaluation index system for rural ecological civilization construction level was constructed based on farmers' satisfaction, then an empirical factor analysis was conducted in Jiangsu Province.[Results] The farmers' satisfaction at the rural ecological civilization construction scored 3.683 6 in Jiangsu Province. Though the construction level was graded as "good" class, there still some room to be further improved. Crucial constraints on the improvement of rural ecological civilization construction level included the rural ecological environment, rural ecological economy.[Conclusion] The evaluation index system of the rural ecological civilization construction level based on the farmers' perspective and the factor analysis are reasonable and feasible.