[Objective] To provide scientific basis for the water pollution control of Hanjiang, the estimation based on the temporal and spatial distribution of non-point source pollution in Jinmen region of Hanjiang watershed was conducted.[Methods] The export coefficient model and the equivalent standard pollution index method were used to illustrate the temporal and spatial distribution and estimated the loads of the non-point source pollution in Jinmen region of Hanjiang watershed, including TN, TP, COD and NH₃-N.[Results] Rural domestic wastes, livestock breeding, rainfall, land use and urban runoff are the major sources of non-point pollution. Based on the pollution stress, spatial distribution of the pollution source load of different cities was found in the following order:Zhongxiang City > Shayang County > Dongbao District > Duodao District. The contribution of different pollutant sources had a rank of urban runoff < rainfall < land use < rural domestic wastes < livestock breeding.[Conclusion] The poor management of pollutants, particularly of the sewage (none or inappropriate treatment), might be the prominent reason of river eutrophication.