[Objective] Land use change and its response to human activities were analyzed comprehensively in Bailongjiang Watershed of Gansu Province during 1990-2014, to provide reference for land use optimization in the future.[Methods] Based on the land use data interpreted from the remote sensing images of 1990, 2002 and 2014, this paper calculated the rate of land use change, the direction of land use transformation and the degree of land use in Bailongjiang watershed of Gansu Province during 1990-2014.[Results] The integrated dynamic degree of land use of the whole watershed increased from 10.24% to 17.59%. The change of land use types was mainly in grassland, arable land and forestland of the watershed during 1990-2014. Comprehensive change index of land use degree of all districts ranged from 1.38~6.65 during 1990-2002 to -15.43~0.17 during 2002-2014.[Conclusion] It proved that land use by human activities had converted from simple development to ecology prone one. Land use shifted from the types of destructiveness, extensive-type and low efficiency to the ones of conservation, intensive-ways and high efficiency in Bailongjiang watershed of Gansu Province during 1990-2014.