[Objective] The coupling link between the urbanization of Lanzhou City and ecosystem service value was analyzed quantitatively to provide scientific basis for appropriate land use in the process of urbanization in Lanzhou City.[Methods] The ecosystem service value and urbanization level in Lanzhou City were constructed and the entropy method was adopted to standardize the model. A coupled and coordinated model between urbanization and ecosystem service value was established based on the data collected from 2004 to 2014 in Lanzhou City. The relationship between urbanization and ecosystem service value, as well as the land use pattern of urbanization, were analyzed.[Results] (1) The urbanization level of Lanzhou City increased from 0.585 1 in 2004 to 0.659 6 in 2014, with an increment of 0.074 5 in 11 years, and annual rate of 0.006 8. And the overall trend of urbanization was increasing. (2) The value of Lanzhou ecosystem services increased from 8.342 billion yuan in 2004 to 9.502 billion yuan in 2014, with an increment of 1.178 billion yuan, and an average annual rate of 107 million yuan, it also showed an increasing trend.[Conclusion] The coupling relationship between urbanization and ecological service value of Lanzhou City in 2004-2014 was an inverted U shape. The overall coupling coordination degree was in moderate coordination state, and the coordination peak occurred in 2009-2012.