[Objective] The aim of study was to analyze the effects of forest ecological service value transfer from upstream in Hunhe River basin on water supply in Shenyang City cluster, which might contribute to make forest ecological compensation standard of the river basin.[Methods] From the perspective of forest ecological service transfer, forest ecological service value transfered from upstream were calculated with ArcGIS 9.3 buffer and overlay analysis platform from 2010 to 2014, and then it studies the effects of forest ecological service on water supply in Shenyang City cluster were studied using the path analysis.[Results] Forest ecological service value transfered from the upstream in Hunhe River basin reached 5.647 billion RMB from 2010 to 2014. Forest ecological service of the water conservation and the soil conservation in the upstream had impacted on the water supply in Shenyang City cluster by surface water, groundwater and chemical oxygen demand(COD).[Conclusion] Forest ecological service value transfer in the upstream generates positive externality, which comprehensively influences on the water supply in Shenyang City cluster by direct effect and indirect effect from multiple factors.