[Objective] A systematic analysis of the impact of meteorological elements on soil moisture in karst area of Guizhou Province was conducted to provide theoretical reference for the prediction of soil moisture, restoration of ecological environment and the optimization of agricultural industrialization in this karst area.[Methods] The effects of meteorological factors on soil moisture were analyzed using two divisional dataset. One dataset is that had increased daily soil moisture and air temperature, another dataset is that had arose soil moisture but declined air temperature. Meteorological factors had daily soil moisture, precipitation, temperature, surface temperature, relative humidity, wind speed and sunshine duration of 10 soil moisture automatic observation stations in karst area of Guizhou Province from 2011 to 2015.[Results] ① The effect of different meteorological factors on the daily soil moisture was lagged to some extent and its lagging time were 10~30 days. ② Precipitation, temperature and surface temperature were the main influence factors of soil water change, having correlation coefficients of 0.66~0.95. The effects of wind speed on soil moisture change were generally weak in majority of agroclimatic zones, its correlation coefficients were less than 0.40. The correlation coefficients of radiation, relative humidity and soil moisture were greater than 0.60 when air temperature was declined. ③ High preceding air temperature and low previous cumulative precipitation were followed by reduced lagging days of precipitation, but the lagging days of air temperature and surface soil temperature were prolonged meanwhile. ④ Precipitation played a major role in the daily soil moisture changes. Changes of other meteorological factors cannot change the downward trend of daily soil moisture when accumulative precipitation was more than 22.4~135 mm in arising stage of air temperature, or when it was greater than 11.2~54.7 mm in declining stage. ⑤ The model between soil moisture and meteorological factors had small (<5.00%), can reflected accurately the influences of meteorological factors on soil moisture.[Conclusion] The response of 10 cm daily soil moisture to climatic factors in Guizhou karst region was lagged. The differences of the lagging time and the correlation coefficient between meteorological elements and soil moisture were mainly caused by stage differences of agroclimatic zones, meteorological factors and daily soil moisture changes.