[Objective] To study the effects of paclobutrazol(PP₃₃₃) and rooting powder(GGR₆) on chlorophyll fluorescence parameters of Cerasus humilis, in order to provide scientific and theoretical basis for strong seedling cultivation techniques of C. humilis seedlings.[Methods] Single factor analysis was used to study the 1-year-old C. humilis seedlings, which were respectively dipped into PP₃₃₃ and GGR₆ with a concentration of 0, 50, 100, 150 and 200mg/L, and the fluorescence was measured by PMA2500.[Results] With the increasing concentration of PP₃₃₃ and GGR₆, the electron transfer efficiency (ETR), actual photochemical quantum efficiency (ΦPSⅡ), photochemical quenching (q_P) and maximum photochemical efficiency (F_v/F_m) were increased, while the non-photochemical quenching(q_N) decreased.[Conclusion] The best plant growth regulator was GGR₆, and the optimal concentration was 200 mg/L. With the same concentration, the effect of GGR₆ on the fluorescence parameters of C. humilis seedlings was better than that of PP₃₃₃.