[Objective] The land use change and its impact on the ecosystem service value in Shandong Province were analyzed to provide guarantee for the regional environmental protection and food security.[Methods] The data of land use types of Shandong Province in 1995, 2005 and 2015 was obtained by using the GIS technology. Then, combined with the relevant evaluation model, the spatial and temporal dynamic evaluation and characteristics of the ecological service values were analyzed in the research area.[Results] ① The land use type in Shandong Province were mainly the cultivated land. Areas of cultivated land, forest land, grassland and unused land were all reduced, while the construction land and water area continued to increase during the period from 1995 to 2015, and the construction land was mainly transformed from the cultivated land. ② From 1995 to 2015, the total value of the ecosystem service in the research area decreased by 6.62×10⁹ Yuan, with a change rate of -2.07%. In those years, the ecological service value of hydrological regulation increased rapidly, while the value of other individual ecological service decreased. Spatially, the value of ecosystem services varied greatly, showing a high spatial distribution in Ludong and Lunan areas, while a low distribution in Luzhong and Luxi areas. The ecological benefited area was small, and the ecological impairment region was widely distributed with an increasing trend. ③ The value of ecosystem services in Shandong Province was inelastic, and the ecological value coefficient adopted was reliable.[Conclusion] Efforts should be made to optimize the land use structure and layout in the future, and land use change should be comprehensively controlled through planning and other measures, so as to promote the stable development of the ecological environment in Shandong Province.