[Objective] To analyze the dynamic change of the major water bodies in Western Jilin Province in order to understand the current situations of water resource in the area, and to provide strong support for the protection, utilization and sustainable development of water resources.[Methods] We extracted the area and spatial distribution of major water bodies in Western Jilin Province by visual interpreting, based on MODIS images from 2000 to 2017. Moreover, the inter-annual and intra-annual variations of water area and the corresponding impact factors were analyzed.[Results] Since 2000, a northward tendency was showed in Yueliangpao reservoir, Xianghai reservoir and Chagan Lake, and the water area had increased. There was an obvious annual periodic change of the water area. Specifically, relatively larger water areas were found during the rainy season, which were compatible with the seasonal climate. The climate change, especially changes of precipitation had significant impacts on water area. In addition, human activities also played a significant role in promoting the growth of water area.[Conclusion] Affected by climate change and human activities, the water area of the major water bodies in Western Jilin Province has increased since 2000.