[Objective] To study the responses of wine grapes for different nitrogen fertilizer application rates and depths, in order to supply theoretical basis for the nitrogen fertilizer application on wine grapes in the Liangzhou irrigation area of Gansu Province.[Methods] Field experiments were conducted to study the effects of different nitrogen fertilizer application rates and depths on grape yield, nitrogen content of fruit, leaf, stalk and residues of nitrate nitrogen in 0-200 cm soil layers during harvest and the second germination season.[Results] There were no significantly difference in the yield, nitrogen content of fruit, leaf, stalk and residues of nitrate nitrogen in 0-200 cm soil layers during harvest and the second germination season between 10 cm and 30 cm fertilization depths. The effects of different nitrogen fertilizer application rates on yield and nitrogen content of stalk reached significant level, but the effects on nitrogen content of fruit and leaf were not significant. There were no significant difference in yield, nitrogen content of fruit and leaf between high level (300 kg/hm²) and middle level (240 kg/hm²) nitrogen fertilizer treatments. Compared with low levels (180 kg/hm²) nitrogen fertilizer treatment, the yield significantly increased by 28.6% and 24%; The nitrogen content of leaf significantly increased by 17.4%, and 11.3%; and the nitrogen content of stalk significantly increased by 40.7% and 33.0% for high and middle levels nitrogen fertilizer treatment, respectively. Compared with middle and low levels nitrogen fertilizer treatment, residues of nitrate nitrogen in 0-200 cm soil layers for high levels nitrogen fertilizer treatment during harvest and germination season increased by 53.8%, 94.4% and 41.8%, 76.1%, respectively. As to the interactions between nitrogen fertilizer application rates and depths, only the nitrogen content of leaf reached a significant level.[Conclusion] Under the conditions of current experiment, the effect of nitrogen application depth on wine grape was not obvious, due to the influence of soil texture and traditional irrigation. The suitable nitrogen fertilizer application rate was 240 kg/hm² for wine grapes, but there is also a risk of nitrogen loss. Therefore, reasonable fertilization amount and fertilization mode should be determined based on irrigation mode in Liangzhou irrigation area.