[Objective] The effects of different rooting agents on the rooting performance in sowing of stem segments of Eremochloa ophiuroides were studied in order to provide reasonably and effectively technological guidance for Eremochloa ophiuroides' function for soil protection and fast growth plantation.[Methods] Focused on some key experimental aspects in sowing of stem segments, the ratio of rooted stem to sowed stem, rooting time, and survival rate of Eremochloa ophiuroides were measured under different rooting agents treatments to select the best rooting agent and methods.[Results] In terms of rootage efficiency, the reduction of rooting time and survival rate, the indolebutyric acid(IBA, 100 mg/L) was the most efficient rooting agent for sowing of stem segments of Eremochloa ophiuroides. Fabric covering also played a vital part in promoting the rooting efficiency and rootage time reduction after sowing, while there was little effect on the ratio of survival.[Conclusion] Different rooting agents have significant effects on the sowing of stem segments of Eremochloa ophiuroides, indolebutyric acid is the best among them.