[Objective] The ecological environment quality of the Huangshan World Heritage Site was quantitatively evaluated to provide a scientific basis for relevant departments for making development and management decisions.[Methods] Taking the core area and buffer zone of Huangshan World Heritage Site as the study area, greenness, humidity, dryness, and heat index components were extracted based on Landsat satellite images, and the remote-sensing ecological index (RSEI) was constructed by principal component analysis. Geographic information system based spatial analysis and statistical methods were used to quantitatively evaluate the ecological environment quality of the study area.[Results] The value of the RSEI in the study area increased from 0.573 in 1992 to 0.638 in 2017, and the ecological environment quality of protected area showed a significant upward trend. In the three-year data analysis, the RSEI grades of good and medium reached more than 65%, which was dominant, and the overall ecological environment quality in the protected area was good. Among the lands with a poor and poorer RSEI grade, approximately 63.6% was converted into land with a high RSEI grade, and the ecological environment quality in the protected area was significantly improved.[Conclusion] The ecological environment quality of the Huangshan World Heritage Site steadily improved, and the protective measures of relevant departments achieved the initial objectives. It is necessary to further coordinate the balance between development and conservation to achieve sustainable development of Huangshan World Heritage Site.