[Objective] The changes of habitat landscape connectivity and its driving factors in the process of land use of ecological migration were analyzed, in order to provide scientific basis for ecological risk management and sustainability of ecological migration. [Methods] Four eco-migration villages (Fengze, Jinlun, Herun and Quangang) in Yuanzhou District of Ningxia Hui Autonomous Region during the 12th five-year plan period were taken as examples. Based on the high-resolution image data of 2016, Google Earth data (2010) and the second national land survey data (2010)before migration, the landscape differentiation in ecological immigrant village was analyzed by calculating the barrier impact index and the landscape connectivity index, after field verification by GPS. [Results] The landscape connectivity of Fengze and Jinlun villages was declining, while that of Herun and Quangang village was on the rise. The change trend of each immigration village was obvious, but the proportion of extremely high and high connectivity was very small. The driving forces of the landscape connectivity change in ecological immigration villages were mainly human factors. Construction land occupied cultivated land and garden land widely in general, and the average annual growth rate of construction land area in Quangang village and Herun village were up to 27.49% and 21.28%, respectively. In addition, Jinlun, Herun and Quangang village had no reserve land resources, and thus, the ecological pressure was prominent. [Conclusion] The increase of population density in the four eco-migration villages resulted in the obvious occupation of cultivated land for construction and the division of ecological land. The ratio of non-agricultural activity of immigrants was significant, resulting in the abandonment of cultivated land, the fragmentation and isolation of landscape, the instability of habitat landscape and the increase of ecological pressure.