[Objective] The ecological security pattern and its influencing factors were studied in order to provide basis and reference for the construction of ecological security in Zhejiang Province, and ensure the overall economic, social and ecological development of the Yangtze River Economic Zone.[Methods] Based on the economic, social and environmental data and the "pressure-state-response (PSR)"" ecological safety evaluation index system, the spatial-temporal evolution characteristics of ecological security pattern in Zhejiang Province from 2009 to 2018 were analyzed by entropy method. The key factors that revealing the impact of key factors on ecological security were extracted by combining the PSR sub-index and factor analysis method.[Results] ① From 2009 to 2018, the comprehensive eco-security index (ESI) of Zhejiang Province fluctuated slightly. ② From the perspective of space, the ESI of Zhejiang Province has reached the early warning state, showing a spatial pattern of changing from "high in the south and low in the north" to "High in the north and low in the south". ③ From the perspective of time nodes, the best year for ecological security of Zhejiang Province was 2016 (0.123), while the worst year was 2011 (0.077), which were in a relatively safe state and a critical security state respectively. ④ Urbanization, economic development, social development, industrial development and resource condition were the important factors affecting the ecological security pattern in Zhejiang Province.[Conclusion] National and regional planning and policies impact the ecological safety index of Zhejiang Province significantly. In order to fully guarantee the ecological safety and healthy development of Zhejiang Province, it is necessary to continuously increase investment in ecological safety construction, promote the co-administration of five kinds of water, promote intelligent and green manufacturing industry, cultivate a number of typical demonstration sites of ecological civilization in ancient villages and characteristic towns, and improve the ecological environment quality.