[Objective] The land use change in Hami City, Xinjiang Uygur Autonomous Region, over the past 20 years was studied to provide strong support for land use planning and ecological environment protection.[Methods] Based on the GlobeLand 30 datasets in 2000, 2010, and 2020, the spatial-temporal change characteristics of land use in Hami City from 2000 to 2020 were analyzed using a transfer matrix, a slope distribution index, a standard deviation ellipse, and the land use and use center-of-gravity migration.[Results] ① The land use change was obvious in Hami City from 2000 to 2020. The proportion of land use types in 2020 was in the order of unused land > grassland > cultivated land > forest > construction land > water area. Although Hami occupied a large area, the usable oasis area was small. In 2020, the grassland area was 10 226.39 km², and the water area had the smallest proportion, with an area of 295.17 km². In terms of land use type change from 2000 to 2020, grassland had the largest reduction, and the loss area was 671.48 km². It was mainly converted into unused land, forest, and a small amount of cultivated land. The increase in construction land was the largest, with an area of 405.60 km². From 2000 to 2020, the area of forest and cultivated land showed an increasing trend, while the unused land and water areas showed a decreasing trend. The reduction of water area was very small, and the area remained stable. ② From 2000 to 2020, grassland was evenly distributed on the five slope gradients. The cultivated and construction land were distributed in grade Ⅰ and grade Ⅱ slope gradients, and cultivated land was dominant in grade Ⅰ slope gradient. The area of forest was the largest on grade Ⅰ gradient. The distribution area of unused land decreased with the increase in the slope gradient. ③ Compared with 2000-2010, the land use change in 2010-2020 had a more obvious directionality and wider range, and the center of gravity shifted to the northwest.[Conclusion] From 2000 to 2020, the land use change of Hami City had obvious space-time characteristics, and the influence of the slope on the distribution of various types is significant.