[Objective] The seasonal dynamics of soil enzyme activities on the rhizosphere of tropical cloud forests in Limushan National Nature Reserve were quantified in order to provide a scientific basis for improving the ecological functions of soil and water conservation in tropical cloud forests from the perspective of soil enzymology. [Methods] The activity of invertase, urease, catalase, and acid phosphatase in rhizosphere and non-rhizosphere soils was measured. Samples were collected using the shaking off method in the dominant tree species (Cyclobalanopsis championii, Castanopsis fabri, Eurya loquaiana) in tropical cloud forests. Relationships between the seasonal dynamics of soil enzyme activities and soil nutrients were also analyzed. [Results] ① The seasonal dynamics of soil enzyme activities in rhizosphere and non-rhizosphere soils of different tree species varied significantly with season, showed a single peak trend, reached a maximum in August of rainy season. The levels of soil enzyme activities in rhizosphere and non-rhizosphere soils of C. championii, and C. fabri were not significantly different, but were significantly higher than for E. loquaiana. ② The level of enzyme activities in rhizosphere soil were significantly higher than in non-rhizosphere soil for different tree species in tropical cloud forests. These results indicated a positive rhizosphere effect that followed the order of acid phosphatase > urease > catalase > invertase. The rhizosphere effects of acid phosphatase, urease, and invertase were not significantly different among different dominant tree species, while the rhizosphere effects of catalase in C. championii, and C. fabri were higher than in E. loquaiana. ③ The level of soil enzyme activities had a significant positive correlation with soil nitrogen(N), phosphorus (P), potassium (K), and had no significant correlation with soil water content and pH value. [Conclusion] Soil enzyme activities in rhizosphere soil and non-rhizosphere soil in different trees in tropical cloud forests exhibited seasonal dynamics, and the rhizosphere effect was significant. Rhizosphere enrichment occurred for dominant tree species. N, P, and K nutrient limitations may be the dominant controlling factors affecting soil enzyme activity and its seasonal variation.