[Objective] The natural resource space of Liuzhou City, which is located in Southern China with typical karst landform and relatively high development level and density of ecological corridors was accurately divided and a control method was proposed in order to provide a scientific reference for research in other karst areas. [Methods] Based on the spatial superposition method, the INVEST model, and the minimum cumulative resistance model, global scale ecological corridors were extracted, the regional ecological security pattern was constructed, and the precise spatial division of natural resources was determined. [Results] ① The ecological source region was dominated by large woodland with an area of 3 531.24 km², and exhibited an overall increasing trend of fields from the central area to the south and to the north; ② The structure of ecological corridors was distributed in a ring shape that was supported by the main corridors and connected by the minor corridors; ③ According to the finished construction of the ecological security pattern and the natural geographical characteristics of Liuzhou City, the natural resources of this city were divided into four controlling areas: areas where development and construction are forbidden, areas where development and construction are restricted, areas where development and construction were permitted, and areas where development and construction were a priority. Differentiated control methods were proposed in accordance with regional ecological environment problems and spatially dominant functions. [Conclusion] The natural resource space control zone was divided on the basis of the ecological security pattern. The development positioning and goals of each control zone were quite different. Differentiated control must be implemented to promote sustainable development of the region.