[Objective] The wetland ecosystem service value in Hubei Province was studied in order to provide a reference for decision-making regarding wetland protection and restoration, and for human sustainable development in the Yangtze River basin. [Methods] Based on principles of wetland resources, ecology, and economics, 12 indicators were selected to evaluate the wetland ecosystem service value in Hubei Province in 2019 by using market value, reflection engineering, and travel expense methods. [Results] The total value of wetland ecosystem service in Hubei Province reached 1.89×10¹² Yuan in 2019, accounting for 40% of the provincial GDP in 2019. Among the 12 indicators evaluated, the order of values ranked as follows: climate regulation (58.18% of the total value), flood control (16.49%), fresh water products (6.24%), oxygen release (5.50%), hydropower (4.04%), water supply (3.07%), carbon sequestration (2.68%), recreation (2.41%), shipping (1.16%), water quality purification (0.23%), production of raw materials (0.003%), and scientific research and education (0.001%). [Conclusion] Climate regulation, flood control, and fresh water products were the leading ecosystem service values (accounting for 80% of the total value), essentially reflecting most of the important ecological characteristics of wetland ecosystems in Hubei Province.