[Objective] The ecological networks in Nanchang City were constructed scientifically and rationally, and the optimal strategies for the defects of the ecological network were researched, in order to provide a scientific basis for ecological construction and help to guide urban planning for biodiversity conservation.[Methods] Based on morphological spatial pattern analysis (MSPA) and landscape connectivity index, the ecological source was selected, the comprehensive resistance surface was constructed considering natural and human factors, the ecological corridor was extracted by minimum cumulative resistance (MCR), and finally the ecological network of Nanchang City was constructed.[Results] The ecological source areas were mainly distributed in the western, northern and eastern regions with higher habitat quality and better connectivity. The distribution characteristic of resistance value was high in the middle area while low in the fringe areas. The spatial distribution of ecological corridor was uneven and the structure was single. The important corridors identified based on gravity model were mainly distributed in the eastern and northern urban fringe area.[Conclusion] It was suggested to strengthen the protection of core habitat patches, increase ecological sources, repair ecological fracture points and optimize network connection. The combination of MSPA and landscape index can effectively identify ecological sources with good ecological quality and high connectivity. In future, we also need to pay attention to the protection of ecological land and improve the landscape connectivity.