[Objective] The changes of eco-hydrological regime in the source region of Dongjiang River basin were studied, in order to provide a basis for clarifying the objectives of eco-restoration and carrying out eco-restoration measures, and to provide experience for the smooth development of the pilot work of water environment and water ecology monitoring of the Ministry of Water Resources.[Methods] The daily flow data of Xunwu shuibei hydrological station in Xunwu County of Jiangxi Province and Dingnan Shengqian hydrological station in Dingnan County from 1980 to 2020 were selected, to comprehensively evaluate the degree of hydrological change of the basin by using IHA-RVA (indicators of hydrologic alteration, IHA; range of variability approach, RVA) method and hydrologic change degree method.[Results] ① The hydrological change degree of the source region of Dongjiang River was 50.1%, which belonged to moderate change, in which the effect of Xunwu Water was greater than Dingnan Water. ② Among the 32 IHA hydrological indexes, the frequency and delay of high and low flow were the least affected, and the degree of change was 26.3%. ③ The maximum flow change rate was 61.5%, which belonged to moderate change, especially the maximum 1-day flow, transformation times, the mean flow in January, the mean flow in May and other indicators have serious variation. ④ According to the analysis of fish resources change, the fish resources in the source region decreased.[Conclusion] The operation of the reservoir has a great impact on the surrounding ecosystem of the source region of the Dongjiang River, and it is urgent to regulate the flow of the river to coordinate the development of regional economy and ecological environment protection.