[Objective] The spatial and temporal shifts in land use structure and changes in ecosystem service values in Yan'an City of Shaanxi Province from 1990 to 2020 were analyzed, in order to provide scientific references for the effective implementation of regional ecological restoration projects and the designation of policies according to local conditions.[Methods] The land use data of Yan'an City obtained from the interpretation of seven periods of remote sensing images from 1990 to 2020 were used to explore the changes in land use structure and the changes in ecosystem service value (ESV) in the study area based on information entropy and offset share models.[Results] ① The information entropy of land use structure in Yan'an City from 1990 to 2020 decreased from 1.14 Nat to 0.99 Nat, and the order of land use structure increased while homogeneity decreased. ② From 1990 to 2020, the construction land, forest land and water area in Yan'an City were the growth structures. In the northern part of Yan'an City, the competitive advantage of construction land, forest land and water area were obvious. In the central area, the competitive advantage of grassland was obvious. And in the southern area, the competitive advantage of arable land was obvious. From 1990 to 2020, the ESV of Yan'an City increased from 2.375 billion yuan to 3.145 billion yuan, and the growth rate of ESV was characterized by "high in the north and low in the south".[Conclusion] Construction land, forest land and water area are the causes of land use growth in Yan'an City, and the ESV growth rate is consistent with the competitive deviation of land use structure.