[Objective] An ecological safety network at Ningbo City, Zhejiang Province was constructed by identifying important ecological sources and potential ecological corridors in order to provide a reference for rationally guiding urban development and establishing key ecological protection areas. [Methods] The ecological source patches at Ningbo City were extracted by ecosystem service value clustering and the outlier analysis method. The multi-source ecological resistance surface was calculated by the minimum cumulative resistance (MCR). The gravity model was used to identify the importance of potential ecological corridors to establish ecological areas at Ningbo City, which formed an ecological safety network. [Results] ① In 2020, the ecosystem service value for Ningbo City was between 0 and 14 929.95 yuan/m², and the high-value clustering area accounted for 23.28% of the total area of Ningbo City. ② The ecological resistance values were between 1 and 3.775. The areas with higher resistance values were located in downtown Ningbo and Cixi City, and the lower values were located at Fenghua District, Ninghai County, and Xiangshan County. ③ Thirty-six common corridors, five generally important corridors, and four important corridors were identified through the gravity model, and 40 potential ecological nodes were generated. Seven ecological areas at Ningbo City were constructed to form an L-shaped ecological safety network pattern. [Conclusion] Ecological security is an important guarantee for guiding the rational development of cities. It promotes the harmonious coexistence of human and nature by protecting the integrity of regional ecological land, and by developing and maintaining potential ecological corridors.