[Objective] The effects of land use transition on ecosystem service values (ESV) in the Wulong District were analyzed in order to provide a basis for decision-making regarding rational land use, optimal allocation of resources, development and protection, and maximization of ESV in the Wuling Mountain region.[Methods] Based on land use data for the Wulong District in 2010, 2015, and 2020, the effects of land use transition on ecosystem service values in the Wulong District were calculated. The spatial-temporal changes of land use transition on ecosystem service values were analyzed by using a land use transfer matrix, a change contribution rate model, and a cross-sensitivity coefficient.[Results] ① In 2020, high values of ESV in the Wulong District were concentrated in Xiannvshan Street and Baima Town in the middle of the Wulong District, while the low-value areas were concentrated on the east and west sides of the Wulong District and Tukan Town in the middle. ② From 2010 to 2020, the ESV in the Wulong District showed an increasing trend; ③ From 2010 to 2020, ESV change and variation contribution rate of different land use types in the Wulong District consistently followed the order of forest>water>cropland>grassland>desert. ④ The sensitivity coefficient of converting grassland to forest land was the highest, while other land use types were not sensitive to the conversion to grassland.[Conclusion] The increase of forest land and water area can greatly increase the total ESV. We suggest increasing the area of garden land and forest land, and strictly controlling the abandonment of cultivated land during the time when adjustments are made to agricultural practices and structure.