[Objective] The comprehensive value of cultivated land in the North China Plain were estimated, and the value differences and change trends were analyzed, in order to provide a scientific basis for the protection of cultivated land in China, and promote the formation of a new pattern of “multiple integrated” cultivated land protection. [Methods] Based on the actual function of cultivated land and the impacts of artificial cultivation, the value of cultivated land was divided into positive values (economic value, social value, and ecological value) and negative values (environmental impacts of pesticides and chemical fertilizers). The comprehensive value of cultivated land in the North China Plain in 2000, 2010, and 2020 was calculated by the income reduction method, the value substitution method, and the equivalent factor method. [Results] The values of cultivated land in the North China Plain in 2000, 2010, and 2020 were 5.01×10¹², 8.45×10¹², and 6.43×10¹² yuan, respectively. The proportion of the economic value of cultivated land showed a downward trend, and the positive value was greater than the negative value during the study period. Moreover, the distribution of the value among provinces was unbalanced, and was greatly affected by cultivated land area, farming methods, and agricultural product prices. The value of cultivated land in Shandong Povince has always been more prominent, while the value of cultivated land in He’nan Province has fluctuated greatly. The negative value of cultivated land per unit area in each province showed an upward trend. [Conclusion] In 2000, 2010, and 2020, relative to the positive value in the North China Plain, the proportion of economic value showed a downward trend, and the importance of the social value and the ecological value of cultivated land gradually became prominent. The negative value per unit of cultivated land in each province showed large regional differences, and the negative value showed an upward trend, especially in He’nan Province. The negative impacts of agricultural film and chemical fertilizer on cultivated land have not been effectively controlled, and the trend is increasing year by year.